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KMID : 0603820090150030233
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Journal of Experimental & Biomedical Science
2009 Volume.15 No. 3 p.233 ~ p.239
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Photodynamic Therapy for Methicillin-resistant Staphylococcus aureus Using Various Photosensitizer
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Kwon Pil-Seung
Jo Yoon-Kyung
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Abstract
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The aim of this study was to evaluate the photodynamic effect of various photosensitizing agents against methicillinresistant Staphylococcus aureus (MRSA). MRSA was exposed to light from a 632 ㎚ diode laser (15 J/㎠) in the presence of various photosensitizer, such as photofrin, photogem, radachlorine and ALA. In vivo study was performed using ICR mice. Twenty eight mice had a standard wound (100 ㎟) created on the dorsum, and MRSA was inoculated into the wound region. The four groups were classified as follows: (1) the untreated control group (bacteria alone), (2) the bacteria plus light group (15 J/㎠), (3) the bacteria plus photofrin group (kept in the dark), and (4) the photodynamic therapy (PDT) group (bacteria, photofrin, and light). After photofrin (dose 1 mg/kg) injection, the experiemental group was irradiated with 632 ㎚ diode laser (15 J/㎠) for 30 minutes after In vitro results of PDT showed the complete killing of MRSA at the photofrin, radachlorine, and photogem However, ALA-PDT was ineffective on MRSA viability. In vivo results showed that photofrin has therapeutic effect on the wound infection. These results demonstrate that selective lethal photosensitization of MRSA can be achieved using phofrin, photogem and radachlorin. Thus, PDT can inactivate MRSA survival.
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KEYWORD
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Methicillin-resistant Staphylococcus aureus, Photodynamic therapy, Photosensitizer, Diode laser
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